FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAAERAAPEI-207_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAAERAAPEI-207_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34211212
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCCTTGACTGTAGCCCTCGTGGCATGTCAACAATTCAACCTTGTTCC492180.14386511650040343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCCT147400.019.7173791
CCCTCGT162100.018.23866817
TGCCTTG158850.017.8777943
CCTCGTG171650.017.26237918
GCCCTCG171150.017.24854516
CTTGACT166550.017.23666
TAGCCCT203400.017.1092314
TTAAGCG106100.016.6074477
GCCTTGA177700.016.4150374
TGTAGCC203700.016.23091112
GTTGTGC79700.015.8933131
TAAGCGT111100.015.8600378
CCTTGTT204400.015.75611442
TTGACTG156350.015.6456167
CTGTAGC213800.015.57733411
GTGGCAT152500.015.26136322
GATTAAG110050.014.9719225
AGCCCTC200500.014.95396815
CTTGTTC268350.014.94463843
CTAGCCT30750.014.87966513
Produced by FastQC (version 0.11.5)